Journal: Stem Cells International

Article Title: Interleukin-1 β Enhances Umbilical Cord Mesenchymal Stem Cell Adhesion Ability on Human Umbilical Vein Endothelial Cells via LFA-1/ICAM-1 Interaction

doi: 10.1155/2019/7267142

Figure Lengend Snippet: p38 MAPK signaling pathway is involved in IL-1 β -mediated LFA-1 expression in MSCs and MSC adhesion to HUVECs. (a) Immunocytochemistry staining for LFA-1 (green) and DAPI (blue) in MSCs. Cells were treated with p38 MAPK (SB 203580, 5 μ M), AKT (GSK690693, 20 μ M), ERK1/2 (U0126 20 μ M), and JNK (SP600125 20 nM) inhibitor and combined with IL-1 β for 30 minutes (scale bar: 50 μ m). (b) Western blot results of the p38 MAPK and phosphorylated p38 MAPK from the lysates of cells pretreated with IL-1 β at 0, 1, 3, 5, 10, and 20 minutes. (c) Western blot results of the LFA-1 (129 kDa) expression in membrane fractions of MSCs treated with IL-1 β and SB 203580 (SB) or cotreated with both IL-1 β and SB. (d) Quantitative graphs of the Western blot results of LFA-1 expression of (c) ( n = 3, ∗∗ P < 0.01). (e) Representative image of MSC adhesion on HUVECs. MSCs were treated with IL-1 β and inhibitor SB 203580 adhesion to IL-1 β -activated HUVECs or nonactivated HUVECs. MSCs labelled with calcein AM (5 μ M) (green), HUVECs, and MSC cell nuclei were stained with Hoechst 33258 (blue) (scale bar: 50 μ m). (f) Quantitative graphs of the cell adhesion assay results of MSCs treated with IL-1 β and MAPK inhibitor SB 203580 adhesion to nonactivated HUVECs (black bars) or IL-1 β -activated HUVECs (gray bars). Values were the cell number fold change relative to the control group. Data represent mean ± SD ( n = 3, ∗∗∗ P < 0.005, ∗∗ P < 0.01, and ∗ P < 0.05) (N.S.: nonsignificance).

Article Snippet: The membrane was blocked in 5% fish gelatin blocking buffer (Amresco, OH, USA) for 1 hour and then incubated with the anti-human CD11a antibody (GeneTex, USA) at 1 : 4000 dilution, anti-p38 MAPK antibody (Santa Cruz, TX, USA) at 1 : 200 dilution, and phospho-p38 MAPK primary antibodies (Cell Signaling, MA, USA) at 4°C overnight.

Techniques: Expressing, Immunocytochemistry, Staining, Western Blot, Membrane, Cell Adhesion Assay, Control

Journal: Stem Cells International

Article Title: Interleukin-1 β Enhances Umbilical Cord Mesenchymal Stem Cell Adhesion Ability on Human Umbilical Vein Endothelial Cells via LFA-1/ICAM-1 Interaction

doi: 10.1155/2019/7267142

Figure Lengend Snippet: Schematic diagram of IL-1 β signaling pathway in MSC adhesion to HUVECs. The schematic diagram depicts the proposed role of IL-1 β signaling pathway in MSC adhesion to HUVECs. The process of cell adhesion is initiated by IL-1 β through p38 MAPK; induced expression of LFA-1 in MSCs enhances the cell adhesion to IL-1 β -induced ICAM-1 in HUVECs.

Article Snippet: The membrane was blocked in 5% fish gelatin blocking buffer (Amresco, OH, USA) for 1 hour and then incubated with the anti-human CD11a antibody (GeneTex, USA) at 1 : 4000 dilution, anti-p38 MAPK antibody (Santa Cruz, TX, USA) at 1 : 200 dilution, and phospho-p38 MAPK primary antibodies (Cell Signaling, MA, USA) at 4°C overnight.

Techniques: Expressing

Journal: Purinergic Signalling

Article Title: Fibroblast growth factor 2 upregulates ecto-5′-nucleotidase and adenosine deaminase via MAPK pathways in cultured rat spinal cord astrocytes

doi: 10.1007/s11302-020-09731-0

Figure Lengend Snippet: FGF2 increases MAPK phosphorylation. Astrocytes were treated with FGF2 (20 ng/ml). SU5402 (SU; 5 μM), U0126 (10 μM), or SP600125 (SP; 10 μM) was added to medium 30 min before FGF2 treatment. The phosphorylation levels of MAPKs were analyzed by western blotting for 10–360 min (a) or 10 min (b). Representative western blots of ERK/p-ERK (42, 44 kDa), JNK/p-JNK (46, 54 kDa), and p38/p-p38 (43 kDa) (left) and summarized data (right) are shown. *P < 0.05 versus control and #P < 0.05 versus vehicle (Steel test), n = 5. Data are presented as medians (IQRs)

Article Snippet: Materials Antibodies against CD73 (#13160, 1:1000), p44/42 MAPK (#4695, 1:4000), phospho-p44/42 MAPK (#9101, 1:4000), SAPK/JNK (#9252, 1:4000), phospho-SAPK/JNK (#9251, 1:4000), p38 MAPK (#9212, 1:4000), and phospho-p38 MAPK (#9211, 1:1000) were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: Phospho-proteomics, Western Blot, Control